超凈工作臺(tái)標(biāo)準(zhǔn)使用方法及日常維護(hù)

    本文著重對(duì)超凈工作臺(tái)的定義，超凈工作臺(tái)的運(yùn)行原理，應(yīng)用方法及超凈工作臺(tái)的日常使用維護(hù)做一個(gè)詳細(xì)說(shuō)明！國(guó)內(nèi)外讀者均可以方便閱讀本文！

   一、什么是超凈工作臺(tái)呢？

　　超凈工作臺(tái)是一種局部?jī)艋O(shè)備，即利用空氣凈化技術(shù)使一定操作區(qū)內(nèi)的空間達(dá)到相對(duì)的無(wú)塵、無(wú)菌狀態(tài)。使用時(shí)，必須放置在潔凈的空間。其接種數(shù)量不受無(wú)菌空的限制，操作比較簡(jiǎn)便，可以較大幅度地提高工作效率，適用于大規(guī)模生產(chǎn)。為了保證接種成功率，接種時(shí)使用酒精燈，效果更好。

    Clean Bench is a partial purification equipment, namely the use of air purification technology to a certain region of space operations to achieve a relatively dust-free, sterile condition. Used, must be placed in a clean space. The number of its vaccination is not sterile space constraints, the operation is simpler, can be more greatly improve work efficiency, suitable for mass production. In order to ensure the success rate of vaccination, inoculation using alcohol lamp, better.

　　二、超凈工作臺(tái)運(yùn)作原理及應(yīng)用方法

　　超凈工作臺(tái)的優(yōu)點(diǎn)是操作簡(jiǎn)單自如，較為舒適，工作效率高，預(yù)備時(shí)間短，開(kāi)機(jī)十分分鐘以上即可操作，基本上可隨時(shí)使用。在工廠化生產(chǎn)中，接種工作量很大，需要經(jīng)常長(zhǎng)久地工作時(shí)，超凈臺(tái)是很理想的設(shè)備。超凈臺(tái)由三相電機(jī)作鼓風(fēng)動(dòng)力，功率145～260W左右，將空氣通過(guò)由特制的微孔泡沫塑料片層疊合組成的“超級(jí)濾清器”后吹送出來(lái)，形成連續(xù)不斷的無(wú)塵無(wú)菌的超凈空氣層流，即所謂“高效的特殊空氣”，它除去了大于0.3μm的塵埃、真菌和細(xì)菌孢子等等。超凈空氣的流速為24～30m/min，這已足夠防止附近空氣可能襲擾而引起的污染，這樣的流速也不會(huì)妨礙采用酒精燈或本生燈對(duì)器械等的灼燒消毒。工作人員就在這樣的無(wú)菌條件下操作，保持無(wú)菌材料在轉(zhuǎn)移接種過(guò)程中不受污染。但是萬(wàn)一操作中途遇到停電，暴露在未過(guò)濾空氣中的材料便難以幸免污染。這時(shí)應(yīng)迅速結(jié)束工作，并在瓶上作出記號(hào)，內(nèi)中的材料如處于增殖階段，則以后不再用作增殖而轉(zhuǎn)入生根培養(yǎng)。如為一般性生產(chǎn)材料，因極其豐富也可棄去。如處于生根過(guò)程，則可留待以后種植用。

    Clean Bench has the advantage of easy to operate freely, more comfortable, efficient, preparation time is short, the boot can be operated for more than a very minute, basically ready to use. In factory production, inoculation heavy workload, the need for frequent long-term work, the Clean Bench is the ideal equipment. Clean Bench by the three-phase motor to make blast power, power 145 ~ 260W or so, will air through the porous foam by a specially composed piece cascading together a "super filter" after blowing out the formation of a continuous dust-free The ultra-clean air laminar flow bacteria, so-called "high-performance special air", which removed more than 0.3μm dust, fungi and bacteria spores and so on. Ultra-clean air flow rate of 24 ~ 30m/min, which are adequate to prevent possible harassment in the vicinity of air pollution caused by such a flow will not impede the use of alcohol lamp or Bunsen burner burning disinfection of equipment and so on. And in this way the staff operated under sterile conditions to maintain sterile material in the course of the transfer of vaccination is not contaminated. But what if an electrical outage halfway operation, exposed to unfiltered air contaminated material will not be spared. Should provide a speedy end to work and make a mark on the bottle, which is in the materials, such as the proliferation phase, we will no longer be used as a proliferation and transferred to rooting culture. As for the general production of materials, due to the extremely rich can also be disposable. As in the rooting process, can be planted until later use.

　　超凈工作臺(tái)電源多采用三相四線(xiàn)制，其中有一零線(xiàn)，連通機(jī)器外殼，應(yīng)接牢在地線(xiàn)上，另外三線(xiàn)都是相線(xiàn)，工作電壓是380V。三線(xiàn)接入電路中有一定的順序，如線(xiàn)頭接錯(cuò)了，風(fēng)機(jī)會(huì)反轉(zhuǎn)，這時(shí)聲音正常或稍不正常，超凈臺(tái)正面無(wú)風(fēng)(可用酒精燈火焰觀察動(dòng)靜，不宜久試)，應(yīng)及時(shí)切斷電源，只要將其中任何兩相的線(xiàn)頭交換一下位置再接上，就可解決。三相線(xiàn)如只接入兩相，或三相中有一相接觸不良，則機(jī)器聲音很不正常，應(yīng)立即切斷電源仔細(xì)檢修，否則會(huì)燒毀電機(jī)。這些常識(shí)應(yīng)在開(kāi)始使用超凈臺(tái)時(shí)就向工作人員講解清楚，免除不應(yīng)造成的事故與損失。

    Clean Bench power supply to use more three-phase four-wire system, of which 10 lines, connecting the machine casing should then firmly on the ground line, the other three lines are the phase, the working voltage is 380V. Third-line access to the circuit in a certain order, such as pick the wrong end of a thread, wind the opportunity to reverse, when the sound normal or slightly abnormal, Clean Bench front without wind (available alcohol lamp flame observation of static and dynamic, not long-trial), should be promptly cut off the power, as long as any of them as two-phase end of a thread to exchange position were followed, the will be over. Three-phase line, such as access only to two-phase or three-phase there is a similar connection is bad, then the machine sounds very normal, should be immediately cut off the power supply careful maintenance, otherwise they will burn motor. These common sense should be when they start using Clean Bench to explain clearly to staff, should not result in exemption from the accident and loss.

　　超凈工作臺(tái)進(jìn)風(fēng)口在背面或正面的下方，金屬網(wǎng)罩內(nèi)有一普通泡沫塑料片或無(wú)紡布，用以阻攔大顆粒塵埃，應(yīng)常檢查、拆洗，如發(fā)現(xiàn)泡沫塑料老化，要及時(shí)更換。除進(jìn)風(fēng)口以外，如有漏氣孔隙，應(yīng)當(dāng)堵嚴(yán)，如貼膠布，塞棉花，貼膠水紙等。工作臺(tái)正面的金屬網(wǎng)罩內(nèi)是超級(jí)濾清器，超級(jí)濾清器也可更換，如因使用年久，塵粒堵塞，風(fēng)速減小，不能保證無(wú)菌操作時(shí)，則可換上新的。

    Clean Bench at the back or front air inlet below the metal mesh cover within a normal foam piece or non-woven fabrics used to block large particles of dust, should be a routine check, unpick and wash, such as found in foam aging, must be promptly replaced. In addition to outside air inlet, where gas leakage pores, it should be blocked strict, such as patching, plug of cotton and paste glue paper. Table inside the front cover of the metal mesh filter is super, super filter may be replaced, as a result of the use of long years, dust jam, wind speed decreased, can not guarantee aseptic operation, can be replaced with new.

    超凈臺(tái)使用壽命的長(zhǎng)短與空氣的潔凈程度有關(guān)。在溫帶地區(qū)超凈臺(tái)可在一般實(shí)驗(yàn)室使用，然而在熱帶或亞熱帶地區(qū)，由于大氣中含有高量的花粉，或多粉塵的地區(qū)，超凈臺(tái)則宜放在較好的有雙道門(mén)的室內(nèi)使用。任何情況下不應(yīng)將超凈臺(tái)的進(jìn)風(fēng)罩對(duì)著開(kāi)敞的門(mén)或窗，以免影響濾清器的使用壽命。

    Clean Bench length of life related to the degree of air cleanliness. Clean Bench in temperate regions may be in the general laboratory use, but in tropical or subtropical regions, because the atmosphere contains high levels of pollen, dust or more areas, Clean Bench are advised to put a better interior double doors. Any case, Taiwan should not be ultra-clean air inlet hood facing the open door or window, so as not to affect the filter life.

　　無(wú)菌室應(yīng)定期用70%酒精或0.5%苯酚噴霧降塵和消毒，用2%新潔爾滅抹拭臺(tái)面和用具(70%酒精也可)，用福爾馬林(40%甲醛)加少量高錳酸鉀定期密閉熏蒸，配合紫外線(xiàn)滅菌燈(每次開(kāi)啟15分鐘以上)等等消毒滅菌手段，以使無(wú)菌室經(jīng)常保持高度的無(wú)菌狀態(tài)。接種箱內(nèi)部也應(yīng)裝有紫外線(xiàn)燈，使用前開(kāi)燈15分鐘以上照射滅菌，但凡是照射不到之處仍是有菌的。在紫外線(xiàn)燈開(kāi)啟時(shí)間較長(zhǎng)時(shí)，可激發(fā)空氣中的氧分子締合成臭氧分子，這種氣體成分有很強(qiáng)的殺菌作用，可以對(duì)紫外線(xiàn)沒(méi)有直接照到的角落產(chǎn)生滅菌效果。由于臭氧有礙健康，在進(jìn)入操作之前應(yīng)先關(guān)掉紫外線(xiàn)燈，關(guān)后十多分鐘即可入內(nèi)。

    Sterile room should be on a regular basis with 70% alcohol or 0.5% phenol spray dust and disinfected with 2% wiped countertops and appliances Bromogeramine (70% alcohol can also be), with formalin (40% formaldehyde) plus a small amount of high manganese airtight fumigation potassium on a regular basis to meet the ultraviolet sterilization lamps (each to open more than 15 minutes) and so on means of disinfection and sterilization, sterile rooms in order to maintain regular high level of sterility. Vaccination should also be equipped with a UV box inside lights, turn on the lights more than 15 minutes prior to use radiation sterilization, but not of the Department any exposure there is still bacteria. UV light to open longer, it can stimulate oxygen molecules in the air to enter the ozone molecule synthesis, this gas composition has a strong bactericidal effect, you can not directly shine on the corner of ultraviolet sterilizing effect produced. Because ozone unhealthy, on entering the operation should be preceded by turn off the UV lamp off after more than 10 minutes you can enter.

　　在超凈工作臺(tái)上亦可吊裝UV紫外線(xiàn)燈，但應(yīng)裝在照明燈罩之外，并錯(cuò)開(kāi)照明燈平行排列，這樣在工作時(shí)不妨礙照明。若將紫外線(xiàn)燈裝入照明燈罩(玻璃板)里面，這是毫無(wú)用處的，因?yàn)樽贤饩€(xiàn)不能穿透玻璃，它的燈管是石英玻璃，而不是硅酸鹽玻璃制成的。

     In the ultra-clean the table can also be hoisting UV ultraviolet light, but it should be packed in light shade outside, and stagger the lights arranged in parallel, so that at work, without prejudice to lighting. Ruoqiang ultraviolet light into light shade (glass) inside, which is useless, since ultraviolet light can not penetrate the glass, it is a quartz glass tube, rather than made of silicate glass.

　　接種室內(nèi)力求簡(jiǎn)潔，凡與本室工作無(wú)直接關(guān)系的物品一律不能放入，以利保持無(wú)菌狀態(tài)。接種室內(nèi)的空氣與外界空氣應(yīng)絕對(duì)隔絕，預(yù)留的通氣孔道應(yīng)盡量密閉。通氣孔道可設(shè)上下氣窗，氣窗面積宜稍大，需覆上4層紗布作簡(jiǎn)單濾塵。在一天工作之后，可開(kāi)窗充分換氣，然后再予以密閉?？傊?，既要清潔無(wú)塵無(wú)菌，又要空氣新鮮，適宜工作。覆在通氣窗上的紗布應(yīng)經(jīng)常換洗。但是上述種種措施只是理想的設(shè)計(jì)方案，往往不易全面做到，其實(shí)只要嚴(yán)格無(wú)菌操作手續(xù)，在門(mén)窗敞開(kāi)的室內(nèi)，有一超凈臺(tái)的保護(hù)，接種的污染率仍可控制在生產(chǎn)上可以容忍的水平。

　　三、超凈工作臺(tái)的日常維護(hù)

    Third, the daily maintenance of ultra-clean bench

　　超凈工作臺(tái)是一臺(tái)較精密的電氣設(shè)備，對(duì)其進(jìn)行經(jīng)常性的保養(yǎng)和維護(hù)是非常重要的。

    我們?cè)鯓幼龊帽ｐB(yǎng)凈化設(shè)備的最重要的超凈工作臺(tái)工作呢？

　　首先要保持室內(nèi)的干燥和清潔，潮濕的空氣既會(huì)使制造材料銹蝕，還會(huì)影響電氣電路的正常工作，潮濕空氣還利于細(xì)菌、霉菌的生長(zhǎng)。清潔的環(huán)境還可延長(zhǎng)濾板的使用壽命。

　　另外，定期對(duì)設(shè)備的清潔是正常使用的重要環(huán)節(jié)。清潔應(yīng)包括使用前后的例行清潔和定期的熏蒸處理。熏蒸時(shí)，應(yīng)將所有縫隙完全密封。清潔應(yīng)包括使用前后的例行清潔和定期的熏蒸處理。熏蒸時(shí)，應(yīng)將所有縫隙完全密封，如操作口設(shè)有可移動(dòng)擋板封蓋的類(lèi)型超凈工作臺(tái)，可用塑料薄膜密封。

　　超凈工作臺(tái)的濾板和紫外殺菌燈都有標(biāo)定的使用年限，應(yīng)按期更換。

 補(bǔ)充一點(diǎn)：在使用超凈工作臺(tái)的工程中，要注意不要有相關(guān)的震動(dòng)產(chǎn)品，會(huì)影響到工作臺(tái)內(nèi)部的風(fēng)機(jī)使用壽命！

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